来源：风湿病与关节炎,2020,9（4）:1-5.

从JAK-STAT信号通路探讨痹证宁对风寒湿痹型类风湿关节炎的作用机制（基础研究）

何东初^1,2，张　倩²

　　【摘　要】目的：探讨痹证宁对风寒湿痹型类风湿关节炎JAK-STAT信号通路的影响及作用机制。方法：将70只Wistar大鼠按照随机数字表法分为空白对照组，模型对照组，痹证宁低、中、高剂量组（9，18，36 g·kg^-1），甲氨蝶呤组（1 mg·kg^-1）和中西医结合组（甲氨蝶呤1 mg·kg^-1+痹证宁18 g·kg^-1），每组10只。除空白对照组外，其余各组大鼠模拟风寒湿环境，采用Ⅱ型胶原诱导方法制备风寒湿痹型类风湿关节炎大鼠模型，各组大鼠给予相应药物干预。28 d后，观察大鼠关节肿胀度、关节滑膜组织白细胞介素-6（IL-6）含量、PIAS3、P-STAT3蛋白和JAK3、STAT3 mRNA表达情况。结果：痹证宁可显著降低风寒湿痹型类风湿关节炎模型大鼠的关节肿胀度，降低IL-6含量，抑制P-STAT3蛋白、JAK3和STAT3 mRNA过表达，增进PIAS3蛋白表达。结论：痹证宁有抗炎、免疫调节作用，其作用机制可能与抑制JAK-STAT信号通路的过度激活有关。

　　【关键词】　关节炎，类风湿；痹证宁；风寒湿痹；JAK-STAT信号通路

On the Mechanism of Bizheng Ning（痹证宁）on Rheumatoid Arthritis of Wind-cold-dampness Bi Type from JAK-STAT Signal Pathway

HE Dong-chu,ZHANG Qian

　　【ABSTRACT】Objective:To explore the effect of Bizheng Ning（痹证宁）on JAK-STAT signal pathway in rheumatoid arthritis of wind-cold-dampness bi type and its mechanism.Methods:Seventy Wistar rats were randomly divided into a blank control group,a model control group,low,medium and high dose Bizheng Ning groups（9,18,36 g·kg^-1）,a methotrexate group（1 mg·kg^-1）and a combination group（1 mg·kg^-1 of methotrexate + 18 mg·kg^-1 of Bizheng Ning）treated with Chinese and Western medicine.There were 10 rats in each group.Except the blank control group,the other groups of rats were used to establish rheumatoid arthritis models of wind-cold-dampness bi type by the method of typeⅡ collagen induction.The rats in each group were given corresponding medicine intervention.After 28 days,the degree of joint swelling,the content of IL-6 in synovium tissue,PIAS3,the expressions of P-STAT3 protein,JAK3 and STAT3 mRNA were observed.Results:Bizheng Ning can significantly reduce the joint swelling and IL-6 content,inhibit the expressions of P-STAT3 protein,JAK3 and STAT3 mRNA,and increase the expression of PIAS3 protein.Conclusion:Bizheng Ning has anti-inflammatory and immunomodulatory effects,whose mechanism may be related to inhibiting the over activation of JAK-STAT signal pathway.

　　【Keywords】arthritis,rheumatoid;Bizheng Ning（痹证宁）;wind-cold-dampness bi;JAK-STAT signal pathway