来源：风湿病与关节炎,2020,9（3）:1-5,11.

乌头汤对脂多糖诱导大鼠软骨细胞炎症反应的影响

（基础研究）

谢新宇^1,2，梅阳阳³，付长龙^1,2，邱志伟⁴，林　晴^1,2，黄艳峰^1,2，郑春松^1,2

　　【摘　要】目的：基于核转录因子-κB（NF-κB）炎症相关信号通路，观察乌头汤水提物对脂多糖（LPS）诱导体外培养软骨细胞炎症模型的影响。方法：①制备乌头汤水提物；②分离、培养软骨细胞；③噻唑蓝（MTT）法检测乌头汤对LPS诱导软骨细胞活性的影响；采用Western Blot法检测NF-κB p65、IκB激酶复合物（IKK）、磷酸化核因子κB抑制蛋白（pIκB）含量表达。结果：①MTT实验结果显示，乌头汤水提物对LPS诱导软骨细胞的最佳干预时间和浓度分别为24 h和150 μg·mL^-1；②Western Blot检测结果显示，乌头汤水提物100 μg·mL^-1组、150 μg·mL^-1组、200 μg·mL^-1组对LPS诱导的软骨细胞干预24 h后，可调节与炎症相关的NF-κB p65、IKK、pIκB含量表达，尤其以乌头汤水提物150 μg·mL^-1组变化显著（P < 0.01）。结论：乌头汤通过降低LPS诱导的软骨细胞中与炎症相关的NF-κB途径调节因子含量表达，发挥抗炎作用。

　　【关键词】　骨关节炎；乌头汤；软骨细胞；炎症反应；大鼠

Effect of Wutou Tang（乌头汤）on Inflammatory Response of Chondrocytes Induced by Lipopolysaccharide in Rats

XIE Xin-yu,MEI Yang-yang,FU Chang-long,QIU Zhi-wei,LIN Qing,HUANG Yan-feng,ZHENG Chun-song

　　【ABSTRACT】Objective:Based on the signal pathway related to the nuclear transcription factor-κB（NF-κB）inflammation,to observe the effect of water extract of Wutou Tang（乌头汤）on lipopolysaccharide（LPS）-induced inflammatory model of chondrocytes in vitro.Methods:①The water extract of Wutou Tang was prepared;②Chondrocytes were isolated and cultured;③MTT method was used to detect the effect of Wutou Tang on LPS-induced chondrocyte activity;and Western Blot method was used to detect the expression of NF-κB p65,IKK,PIκB.Results:①MTT assay showed that the best intervention time and concentration of Wutou Tang water extract on LPS-induced chondrocytes were 24 hours and μg·mL^-1 respectively;②Western Blot assay showed that after 24 h intervention to the LPS-induced chondrocytes in the 100 μg·mL^-1 group,the 150 μg·mL^-1 group and the 200 μg·mL^-1

 group,the protein expression of NF-κB p65,IKK,PIκB related to inflammation could be regulated especially the 150 μg·mL^-1of Wutou Tang water extract changed significantly（P < 0.01）.Conclusion:Wutou Tang plays an anti-inflammatory role by reducing the expression of NF-κB pathway regulatory factor in LPS induced chondrocytes.

　　【Keywords】osteoarthritis;Wutou Tang（乌头汤）;chondrocyte;inflammatory response;rat