来源：风湿病与关节炎,2019,8（5）:5-8.

药食两用3号方对大鼠热证痛风性关节炎的抗炎抗氧化活性研究（基础研究）

张伯瑞¹，薛崇祥¹，何世勇¹，肖　楠¹，田　源²，郁万刚²，陶　方²

　　【摘　要】目的：研究药食两用3号方对热证痛风性关节炎的作用。方法：将32只SD大鼠随机分为空白对照组、模型对照组、秋水仙碱组和复方干预组，每组8只。除空白对照组外，其余各组均通过注射尿酸钠造成大鼠痛风性关节炎模型，并置于高温环境下制成热证模型，用秋水仙碱和痛风药食两用3号方分别对秋水仙碱组和复方干预组大鼠灌胃给药。检测踝关节周长增加百分比，末次给药后腹主动脉采血，检测大鼠血清中αN乙酰氨基葡萄糖苷酶、β-半乳糖苷酶、超氧化物歧化酶、尿酸、肿瘤坏死因子-α的水平。结果：与模型对照组比较，复方干预组踝关节周长增加百分比、αN乙酰氨基葡萄糖苷酶、β-半乳糖苷酶、尿酸、肿瘤坏死因子-α均明显降低，超氧化物歧化酶明显升高（P < 0.01）。结论：药食两用3号方对热证痛风性关节炎大鼠有降尿酸、抗炎抗氧化的作用。其作用机制可能与降低超氧化物歧化酶活性，抑制肿瘤坏死因子-α、αN乙酰氨基葡萄糖苷酶、β-半乳糖苷酶合成与释放有关。

　　【关键词】　痛风性关节炎；热证型；药食两用3号方；抗炎；抗氧化；大鼠

Study on Anti-inflammatory and Anti-oxidative Activities of Edible-drug Formula 3（药食两用3号方）in Rats with Gouty Arthritis of Heat Syndrome

ZHANG Bo-rui,XUE Chong-xiang,HE Shi-yong,XIAO Nan,TIAN Yuan,YU Wan-gang,TAO Fang

　　【ABSTRACT】Objective:To study the effect of edible-drug formula 3（药食两用3号方）on gouty arthritis of heat syndrome.Methods:Thirty-two SD rats were randomly divided into a blank control group,a model control group,a colchicine group and a compound intervention group,8 rats in each group.Except the blank control group,the other groups were induced gouty arthritis models by injecting sodium urate,and were subjected to high temperature to make heat syndrome models.Colchicine and edible-drug formula 3 were given orally to the colchicine group and the compound intervention group respectively.Detection of percentage increase in ankle circumference was made.After the last administration,the abdominal aorta blood was taken to detect the levels of serum αN hexanyl glucosidase,β-galactosidase,superoxide dismutase,uric acid and tumor necrosis factor-α.Results:Compared with the model control group,the percentage of ankle circumference increase,αN hexyl glucosaminidase,β-galactosidase,uric acid and tumor necrosis factor-α were significantly decreased,and superoxide dismutase was significantly increased in the compound intervention group（P < 0.01）.Conclusion:Edible-drug formula 3 has the effect of reducing uric acid,anti-inflammation and anti-oxidation in rats with gouty arthritis of heat syndrome.Its mechanism may be related to the reduction of superoxide dismutase activity,inhibition of synthesis and release of TNF-α,αN hexanyl glucosaminidase and β-galactosidase.

　　【Keywords】 gouty arthritis;heat synd-rome type;edible-drug formula 3（药食两用3号方）;anti-inflammatory; anti-oxidant;rats