来源：风湿病与关节炎,2023,12（6）:1-5,12.

注射用蜂毒对热痛模型小鼠的痛阈和外周血P物质、β-内啡肽水平的影响（基础研究）

董　泓^1,2，李振彬¹，王志强¹，高囡囡¹，王丽敏¹，郭栋梁¹ 

　　【摘　要】目的：探索注射用蜂毒（BVI）对热痛模型小鼠的镇痛作用及对外周血P物质（SP）、β-内啡肽（β-EP）水平的影响。方法：选择合格昆明小鼠200只，随机分为BVI大剂量（BVI-H）组、BVI中剂量（BVI-M）组、BVI小剂量（BVI-L）组、吗啡组、模型对照组，给药后分别采用热板法和辐射热法建立小鼠热痛模型，观察不同组别给药后不同时间痛阈的动态变化；实验结束后，心脏取血，分离血清，ELISA法检测血清SP、β-EP水平。结果：BVI各剂量组和吗啡组均显示出明显的镇痛作用，BVI在热板法疼痛模型的作用具有剂量依赖性。BVI对小鼠疼痛模型血清SP、β-EP水平有一定影响，BVI各剂量组和吗啡组均对SP显示出下调效应；血清β-EP水平在BVI-L组升高，BVI-M组和BVI-H组则下降。结论：BVI对小鼠热痛模型具有显著镇痛作用，其相关机制可能与抑制内源性疼痛介质传递、增加内源性镇痛介质分泌等有关。

　　【关键词】　疼痛；热痛模型；注射用蜂毒；镇痛；P物质；β-内啡肽；小鼠

Effects of BVI on Pain Threshold and the Levels of SP and β-EP in Mouse Model with Heat Pain

DONG Hong,LI Zhen-bin,WANG Zhi-qiang,GAO Nan-nan,WANG Li-min,GUO Dong-liang

　　【ABSTRACT】Objective:To explore the analgesic effect of Bee Venom for Injection（BVI）on mouse models with heat pain and its effects on the levels of substance P（SP）and β-Endorphins（EP）in peripheral blood.Methods:Two hundred qualified Kunming mice were selected and randomly divided into the high-dose BVI（BVI-H）group,the medium-dose BVI（BVI-M）group,the low-dose BVI（BVI-L）group,the morphine group,and the model control group.After administration,hot plate method and radiation heat method were used to establish mouse models with heat pain,and the dynamic changes of pain threshold at different times in each group were observed after administration.After the experiment,blood was taken from the heart,serum was separated,and the levels of SP and β-EP in serum were detected by ELISA.Results:All dose groups of BVI and the morphine group showed significant analgesic effects,and the effect of BVI on the pain models with hot plate  was dose-dependent.BVI has a certain impact on the levels of SP and β-EP in serum,and all dose groups of BVI and morphine showed a downregulation effect on SP.The level of β-EP increased in the BVI-L group,while decreased in the BVI-M and BVI-H groups.Conclusion:BVI has a significant analgesic effect on mouse models with heat pain,and its related mechanisms may be related to inhibiting the transmission of endogenous pain mediators and increasing the secretion of endogenous pain mediators.

　　【Keywords】 pain;model with heat;BVI;analgesia;SP;β-Endorphins;mouse