来源：风湿病与关节炎,2017,6（9）:5-9.

独活寄生汤含药血清对退变软骨细胞表达miRNA-140的影响

（基础研究）

陈　俊¹，叶锦霞²，林　洁¹，林　婧²，林秋祥³，刘献祥²，吴广文²

　　【摘　要】目的：观察独活寄生汤含药血清对体外培养的大鼠退变软骨细胞表达miRNA-140的影响，探讨独活寄生汤防治骨关节炎的作用机制。方法：4周龄SD大鼠膝关节软骨建立体外培养的软骨细胞，采用Ⅱ型胶原原位杂交法对第3代（F3）软骨细胞进行鉴定。用10 ng·mL^-1白细胞介素-1β诱导F3软骨细胞，复制退变软骨细胞模型。成功后随机分为模型组和独活寄生汤含药血清组，分别在培养24，48，72 h后采用TaqMan real-time PCR法检测各组miRNA-140表达。结果：独活寄生汤含药血清组miRNA-140相对表达量随着干预时间的延长逐渐增加，差异有统计学意义（P < 0.05）。结论：独活寄生汤治疗骨关节炎的部分作用机制是有效促进退变软骨细胞miRNA-140的表达。

　　【关键词】　骨关节炎；独活寄生汤；含药血清；软骨细胞；miRNA-140 

Effects of Serum Medicated with Duhuo Jisheng Tang（独活寄生汤）on the Expression of miRNA-140 in Degenerated Cartilages

CHEN Jun,YE Jin-xia,LIN Jie,LIN Jing,LIN Qiu-xiang,LIU Xian-xiang,WU Guang-wen

　　【ABSTRACT】Objective:To observe the effects of serum medicated with Duhuo Jisheng Tang（独活寄生汤）on the expression of miRNA-140 in degenerated cartilages cultured in vitro in order to explore the mechanism of Duhuo Jisheng Tang treating osteoarthritis.Methods:Chondrocytes were cultured in vitro in four-week old SD rats and the third generation（F3）chondrocytes were identified by in situ hybridization of typeⅡcollagen.F3 chondrocytes were induced by 10 ng·mL^-1 IL-1β,and the degenerated chondrocytes were duplicated.The rats were divided into a model group and a drug serum group.TaqMan real-time PCR method was used to detect the expression of miRNA-140 of each group after being cultured for 24,48 and 72 h respectively.Results:The expression time of miRNA-140 in serum medicated with Duhuo Jisheng Tang increased gradually with the prolonged time,and the difference was statistically significant（P < 0.05）.Conclusion:The mechanism of Duhuo Jisheng Tang in the treatment of osteoarthritis is effective to promote the expression of miRNA-140 in degenerated cartilage.

　　【Keywords】 osteoarthritis;Duhuo Jisheng Tang（独活寄生汤）;medicated serum;chondrocytes;miRNA-140