来源：风湿病与关节炎,2023,12（9）:6-10,26.

润燥灵对干燥综合征患者外周血单个核细胞白细胞介素-17调控作用研究（基础研究）

胡小钰，罗泽红，黄芳琴，曾　苹，马武开

　　【摘　要】目的：探讨解毒化瘀药润燥灵对干燥综合征患者外周血单个核细胞（PBMCs）白细胞介素-17（IL-17）的调控作用。方法：选取符合诊断标准的干燥综合征患者30例，抽取静脉血20 mL，分离提取PBMCs，加入10 ng·mL^-1的白细胞介素-1β（IL-1β）、10 ng·mL^-1的IL-6、1 ng·mL^-1的转化生长因子-β（TGF-β）、1 ng·mL^-1的肿瘤坏死因子-α诱导CD4⁺ T细胞分化成Th17细胞。将细胞分为空白对照组，IL-17刺激组，IL-17抑制组和润燥灵高、中、低剂量组。采用CCK-8检测润燥灵对干燥综合征患者Th17细胞的抑制率；ELISA法检测IL-17A、IL-17F、IL-23、TGF-β的分泌，qRT-PCR检测IL-17A、IL-17F mRNA的表达水平。结果：CCK-8检测结果显示，经润燥灵处理24 h、48 h后，Th17细胞增殖能力下降。ELISA法检测结果显示，与IL-17刺激组比较，IL-17抑制组及润燥灵高、中剂量组IL-17A、IL-17F、IL-23浓度明显下降，差异有统计学意义（P < 0.05）。RT-PCR检测结果示，与IL-17刺激组比较，IL-17抑制组和润燥灵各剂量组IL-17A、IL-17F mRNA表达量显著下调，差异有统计学意义（P < 0.05）。结论：中药润燥灵能够抑制IL-17A、IL-17F、IL-23的分泌，增加TGF-β抑炎因子的分泌，从而抑制Th17细胞炎症反应。

　　【关键词】　干燥综合征；润燥灵；解毒化瘀药；CD4⁺ T细胞；Th17细胞；白细胞介素-17

On the Regulatory Effect of Runzaoling（润燥灵）on Interleukin-17 in Peripheral Blood Mononuclear Cells of Patients with Sjögren's Syndrome

HU Xiao-yu,LUO Ze-hong,HUANG Fang-qin,ZENG Ping,MA Wu-kai

　　【ABSTRACT】Objective:To explore the regulatory effect of the detoxifying and stasis-resolving drug Runzaoling（润燥灵）on interleukin-17（IL-17）in peripheral blood mononuclear cells（PBMCs）of patients with Sjögren's syndrome.Methods:Thirty patients with Sjögren's syndrome who met the diagnostic criteria were selected.20 mL of venous blood was drawn,from which PBMCs were separated and extracted.IL-1β（10 ng·mL^-1）,IL-6（10 ng·mL^-1）,TGF-β（1 ng·mL^-1）and tumor necrosis factor-α（1 ng·mL^-1）were added to induce CD4⁺ T cells to differentiate into Th17 cells.The cells were divided into a blank control group,the IL-17 stimulation group,the IL-17 inhibition group,and the high,medium,and low dose groups of Runzaoling.CCK-8 was used to detect the inhibitory rate of Runzaoling on Th17 cells in patients with Sjögren's syndrome;ELISA was used for the detection of the secretion of IL-17A,IL-17F,IL-23 and TGF-β;and qRT-PCRwas used to detect the expression level of IL-17A and IL-17F mRNA.Results:CCK-8 results showed that after being treated with Runzaoling for 24 and 48 hours,the proliferation ability of Th17 cells decreased.The ELISA results showed that compared with the IL-17 stimulation group,the concentrations of IL-17A,IL-17F,and IL-23 in the IL-17 inhibition group and the high and medium dose groups of Runzaoling decreased significantly,with a statistically significant difference（P < 0.05）.The RT-qPCR detection results showed that compared with the IL-17 stimulation group,the expression of IL-17A and IL-17F mRNA in the IL-17 inhibition group and each dose group of Runzaoling significantly down-regulated,with a statistically significant difference（P < 0.05）.Conclusion:Chinese medicine Runzaoling can inhibit the secretion of IL-17A,IL-17F,and IL-23,and increase the secretion of anti-inflammatory factors TGF-β,to inhibit the inflammatory response of Th17 cells.

　　【Keywords】 Sjögren's syndrome;Runzaoling（润燥灵）;detoxifying and blood stasis resolving drug;CD4⁺ T cell;Th17 cell;interleukin-17