来源：风湿病与关节炎,2021,10（6）6-9.

类风湿关节炎患者外周血调节性T细胞分泌白细胞介素-35水平与其发病的相关性研究（临床研究）

李　丹，石　红，孔祥红，张景利

　　【摘　要】目的：探究类风湿关节炎患者外周血调节性T细胞（Treg细胞）分泌白细胞介素-35（IL-35）水平与其发病的相关性。方法：选取类风湿关节炎患者70例作为观察组，健康体检者70例为对照组。采集2组外周血，检测Treg细胞数量、Foxp3荧光强度、IL-35水平。同时将观察组患者按照28个关节疾病活动度评分（DAS28评分）分为不同的组，对各组Treg细胞、Foxp3荧光强度、IL-35水平进行检测。结果：观察组患者外周血中Treg细胞高于对照组，IL-35水平低于对照组，差异有统计学意义（P < 0.05）；2组Foxp3荧光强度比较，差异无统计学意义（P > 0.05）。各DAS28评分组RA患者的Treg细胞、IL-35水平比较，差异有统计学意义（P < 0.05），且DAS28评分越高，Treg细胞数量和IL-35水平越低；各DAS28评分组RA患者Foxp3荧光强度比较，差异无统计学意义（P > 0.05）。结论：类风湿关节炎患者外周血中Treg细胞数量明显上升，IL-35水平显著下降。由于Treg细胞功能缺陷，降低了负调控能力。

　　【关键词】　类风湿关节炎；外周血调节性T细胞；白细胞介素-35；相关性

The Correlation Between the Level of IL-35 Secreted by Treg Cells in Peripheral Blood and the Pathogenesis of Rheumatoid Arthritis

LI Dan,SHI Hong,KONG Xiang-hong,ZHANG Jing-li

　　【ABSTRACT】Objective:To investigate the correlation between the level of IL-35 secreted by Treg cells in peripheral blood and the pathogenesis of rheumatoid arthritis（RA）.Methods:Seventy RA patients were selected as an observation group and 70 healthy physical examinees were selected as a control group.The peripheral blood of both groups was collected.The number of Treg cells,the fluorescence intensity of Foxp3 and the level of IL-35 were measured.At the same time,the patients in the observation group were divided into different groups according to the DAS28 score,and the Treg cells,Foxp3 fluorescence intensity and IL-35 levels in each group were detected.Results:The level of Treg cells in peripheral blood of the observation group was higher than that of the control group,and the level of IL-35 was lower than that of the control group（P < 0.05）;There was no significant difference in the fluorescence intensity of Foxp3 between the two groups（P > 0.05）.The difference of Treg cells and IL-35 levels in RA patients with different DAS28 scores was statistically significant（P < 0.05）,and the higher the score of DAS28,the lower the number of Treg cells and IL-35 levels;There was no significant difference in the fluorescence intensity of Foxp3 between RA groups with different DAS28 scores（P > 0.05）.Conclusion:The number of Treg cells in the peripheral blood of RA patients increased significantly,and the level of IL-35 decreased significantly.Because of the deficiency of Treg cells,the negative regulation ability was reduced.

　　【Keywords】 rheumatoid arthritis;Treg cells of peripheral blood;IL-35;correlation